Scientific Technological Platforms
Analytics Unit
Standardized target analytical services in the AU
CYP450 enzyme activity assessment
Description
The method involves incubation of a biological sample with a model substrate cocktail. The phase I metabolites formed are then determined using a UPLC-MS/MS methodology.
Application
In vitro evaluation of the main activities of CYP450, one of the main enzymes in the Phase I metabolic mechanism.
Evaluation of the enzymatic activity of the UGTs.
Description
The method involves the incubation of a biological sample with a model substrate cocktail. The phase II metabolites formed are then determined using a UPLC-MS/MS methodology.
Application
In vitro evaluation of the main activities of UGTs, one of the main enzymes in the Phase II metabolic mechanism
Bile acid profiles
Description
Determination of 31 conjugated and unconjugated bile acids in serum, plasma and tissue samples using a UPLC-MS/MS-based methodology.
Application
Study of bile acid metabolism in physiological and pathological situations, especially in toxicological studies.
Determination of biomarkers of oxidative stress
Description
Determination of oxidative stress biomarkers of protein, lipid and DNA damage in serum, tissue or cell culture samples using a methodology based on UPLC-MS/MS.
Application
Reliable and rapid detection of homeostasis and oxidative damage at different biological levels.
Determination of eicosanoids
Description
UPLC-MS/MS methodology for the determination in tissue and serum samples of this family of compounds, which include leukotrienes, isoprostanes, prostaglandins and thromboxanes, known biomarkers of lipid peroxidation and inflammation.
Application
Study of endogenous oxidative stress and inflammatory state.