Microscopy Unit

Observation and imaging of samples with histological staining
Sample processing for fluorescence microscopy
- Fluorescent labelling
- Immunofluorescence
- Observation of samples and fluorescence microscopy imaging
- Observation of samples and confocal microscope imaging
Sample processing for transmission electron microscopy
- Chemical fixation
- Counteracting (osmium tetroxide and uranyl acetate)
- Dehydration in the gradient of alcohols (ethanol, methanol)
- Embedding in epoxy resin (EPON 812) or acrylic resin (Lowicryl K4M)
Ultramicrotomy
- Obtaining semi-thin sections (0.5-2 microns) for optical microscopy and ultra-thin sections (60-100 nm) for electron microscopy.
- Contrasting these sections
Immunopreparation for electron microscopy (immuno-gold)
- Preembedding
- Post-embedding
Low-temperature preparation of biological samples for electron microscopy
Cryosubstitution
Low-temperature inclusion
Sample observation and image capture under transmission electron microscopy
Consulting services
- Preliminary study of the proposal
- Experimental design and elaboration of specific protocols for each type of biological material and the specific aspects to be studied.
- Advice for the continuity of the study.
- Full collaboration in the project.

REQUEST FOR SERVICES